A highly sensitive nanobiosensor based on aptamer-conjugated graphene-decorated rhodium nanoparticles for detection of HER2-positive circulating tumor cells

Abstract Assessment of human epidermal growth factor receptor-2 (HER2) tumor marker status is an impressive in screening, diagnosing and monitoring breast cancer (BC). The electrochemical biosensor a revolutionary method diagnosis, which used this research to detect HER2 + circulating cells. activity, size, shape, morphology the synthesized nanomaterials were analyzed. hybrid nanocomposite established by coupling reduced graphene oxide nanosheets (rGONs) rhodium nanoparticles (Rh-NPs) on surface graphite electrode resulted improved area, biocompatibility. electrode-based aptasensor (g-aptasensor) demonstrated exceptional performance against HER2-overexpressed SKBR3 cells, with linear dynamic range 5.0 10.0 × 10 4 cells/mL, analytical limit detection (LOD) as low 1.0 cell/mL, quantification (LOQ) 3.0 cells/mL. G-rich DNA aptamers can fold into intermolecular G-quadruplex, specifically bind target molecule. Consequently, advantages highly efficient platform include broad range, high specificity, selectivity, stability, reproducibility, cost. These characteristics indicate that fabricated nanobiosensor has potential for use detecting level care BC patients clinical diagnosis.